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Related post: line inhibited the proliferation of the immunogen to interleukin-2 (IL-2). Cell distribution studies demonstrated that the antigen defined by 7D4 was present on activated T and B lymphocytes, but was undetectable on normal murine lymphoid cells. Competition binding studies indicated that 7D4 failed to inhibit the binding of radiolabeled IL-2; however, 7D4 did precipitate radiolabeled IL-2 from detergent Buy Calcium Carbonate extracts of activated T cells that had been pulsed with IL-2. It is therefore likely that 7D4 detects an epitope on the IL-2 receptor distal to the ligand binding site or another molecule that physically associates with the receptor. A similar approach was used to prepare a murine monoclonal antibody (5C3) to a cloned line of guinea pig alloreactive T cells . 5C3 inhibited IL-2 driven proliferation as well as antigen and mitogen induced proliferation, but did not appear to define the guinea pig IL-2 receptor because it did not inhibit the binding of IL-2 and because T cell blasts that had been stripped of the antigen defined by 5C3 were not deficient in their expression of the IL-2 receptor. However, a role for the 5C3 bearing molecule in IL-2 driven growth was suggested by the observation that the 5C3-negative blasts were unable to proliferate to IL-2. The use of reagents Generic Calcium Carbonate such as 7D4 and 5C3 should greatly facilitate the analysis of the role of IL-2 as a universal growth hormone for T cells. These Purchase Calcium Carbonate reagents are also attractive candidates for in vivo therapeutic use in attempts to selectively modulate or abrogate an ongoing immune response. _^___ PHS 6040 (Rev. 1/83) 20-23 GPO 895-100 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00225-02 LI PERIOD COVERED October 1, 1982 to September 30, 1983 TITLE OF PROJECT (80 character! or lest. Title must fit on one line between the borders.) The effects of UV irradiation on IL-1 production by keratinocytes & macrophages PRINCIPAL INVESTIGATOR (List other professional personnel on subsequent pages.) (Name, title, laboratory, and Institute affiliation) Ira Green, M.D. , Sr. Investigator, Laboratory of Immunology, NIAID COOPERATING UNITS (if any) Thomas Luger, M.D., Second Department of Dermatology, University of Vienna Medical School, Vienna, Austria LAB/BRANCH Laboratory of Immunology INSTITUTE AND LOCATION NIAID, NIH, Bethesda, Maryland 20205 TOTAL MANYEARS: 1.75 PROFESSIONAL: 1.50 OTHER: .25 CHECK APPROPRIATE BOX(ES) □ (a) Human subjects □ (a1) Minors □ (a2) Interviews □ (b) Human tissues [3 (c) Neither SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.) Lymphokines are substances produced by lymphocytes and monocytes that have important functions in lymphoid cell interactions and regulation . One lymphokine, IL-1 , was previously found to be produced by monocytes and macrophages. Recently, however, epidermal keratinocytes were also found to be able to produce IL-1. Since ultraviolet irradiation (W) was found to have deleterious effects on several immune functions, both in vivo and in vitro, we investigated the effects of UV on IL-1 production by keratinocytes in vitro and in vivo and in normal and malignant macrophages in vitro. Contrary Cheap Calcium Carbonate to our expectations, UV induced increased production of IL-1 in all these experiments. These results do not support the idea that the UV induced defect produced in antigen presenting cells is the result of decreased IL-1 production. Increased IL-1 production by keratinocytes after UV may also have important systemic consequences. PHS6040 (Rev. 1/83) 20-24 GPO 895-100 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER Z01 AI 00226-02 LI PERIOD COVERED October 1, 1982 to September 30, 1983 TITLE OF PROJECT (80 characten or leei. Title must fit on one line between the borders.) Rabbit Allotypes: Structure, Organization and Regulated Expression of Ig Genes PRINCIPAL INVESTIGATOR (List other professional personnel on subsequent pages.) (Name, title, laboratory, and institute affiliation) Rose G. Mage, Ph.D., Sr. Investigator, Laboratory of Immunology, NIAID COOPERATING UNITS (if any) E.P. Reddy, LCMB/NCI LAB/BRANCH Laboratory of Immunology INSTITUTE AND LOCATION NIAID, NIH, Bethesda, Maryland 2020b TOTAL MANYEARS: 4.0 PROFESSIONAL; 2.0
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